At Diffuse Bio we’re building generative AI for protein therapeutics. Our team has been behind breakthroughs in AI protein design for the past 6 years, including the first experimental validation of AI-generated proteins and diffusion models for protein structure and sequence. We’ve built the first entirely AI computational nanobody design platform and are testing designs in lab now. 

Our goal is to build AI systems that can design protein therapeutics for the most challenging and high-value targets. We see enormous potential for AI to revolutionize drug discovery and expand the set of diseases we can treat. 

We are looking for candidates who are excited about the opportunity to join the founding team and play an expanding role in the company.

**Full-time, part-time, and internships available. We’re located in San Carlos.

The role:**

• Run molecular biology and protein engineering assays, assist in running high-throughput protein binding screens

• Work with the computational team on experimental design to assay AI-designed proteins.

• Located in San Carlos (in-person)

Responsabilities:

• Molecular biology assays (PCR, cloning, transformation)

• Protein expression and characterization (bacteria and/or yeast protein expression, IMAC, stability characterization, ELISA)

• DNA library preparation, cloning

• Run in vitro display screens (phage and/or yeast display)

• NGS (Next-gen sequencing) data collection and analysis

Ideal background:

• Highly organized and methodical, detail-oriented and takes initiative

• Significant experience in designing, running, debugging standard molecular biology and protein engineering assays. 

• 2-3 years industry experience in a protein engineering position or Graduate degree in Biology, Bioengineering, Biochemistry, or related fields

Pluses:

• Familiar with Benchling and Quartzy

• Deep knowledge of physics, math, molecular biology, chemistry, etc.

• Graduate degree in Biology, Bioengineering, Biochemistry, or related fields.

• Experience with high-throughput assays (yeast display, phage display)

• Experience with surface plasmon resonance (SPR) or bilayer interferometry (BLI)  or circular dichroism (CD) spectroscopy

What we offer:

• The opportunity to join the founding team and play a critical and expanding role in shaping the company.

• The opportunity to work on cutting-edge AI with leading researchers from top institutions.